The bus series usually includes the main cast plus some of the producers or guests cosplaying or dressing up in drag and playing mini games in a bus going around popular Tokyolocations. When one of the players loses a mini-game, he is dropped off in a public location and forced to walk back to the studio alone and looking quite absurd. Past themes include female anime characters, kogals, SM queens, and Pink Lady (Japanese pop music idols of the late 1970s).

One memorable moment was during the Anime Bus Tour, when Yamasaki (dressed as Arale from Dr. Slump) was dropped off and told to buy take-out spaghetti for the entire cast on his way back. He attempted to walk back without buying any, but on his way, he ran into fellow contestant Miyasako of the comedy duo Ameagari Kesshitai, who had also been dropped off (dressed as Kekkō Kamen). They bought the spaghetti together. Upon arriving at the studio, Miyasako revealed that Yamasaki had tried to return without buying the food. Appalled and outraged, Matsumoto ordered him to go buy spaghetti in Italy -- specifically, to go to the "very tip of its heel," referring to the country's distinct boot-like shape. A few weeks later, Yamasaki was forced to fly to Lecce, Italy to buy a plate of spaghetti from a restaurant, all the while still dressed as Arale.

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